Glut4 And Diabetes
To gain further insight into the mechanisms underlying muscle insulin resistance, the influence of obesity and type 2 diabetes on glut4 immunoreactivity in slow and fast skeletal muscle fibers was studied. through a newly developed, very sensitive method using immunohistochemistry combined with morphometry, glut4 density was found to be significantly higher in slow compared with fast fibers in. F and g: confocal imaging of glut4 and plasma membrane marker caveolin-3 (cav3) was performed on clamped (insulin-stimulated) gastrocnemius cryosections. quantification of sarcolemmal glut4 was performed by imagej. n = 11–15/group. h: vastus lateralis homogenates were applied to a 4–12% sds-page. western blotting was performed for hkii and. Total glut4 content in skeletal muscle from individuals with type 2 diabetes is normal; however, recent studies have demonstrated that translocation of glut4 to the plasma membrane is decreased in response to insulin stimulation. Insulin and amp-activated pr...